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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
09/12/2021 |
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Data da última atualização: |
09/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZORZI, F. M.; ZAFALON, L. F.; SANTOS, F. B.; NASCIMENTO, T. G.; BASÍLIO JUNIOR, I. D.; MAMIZUKA, E. M.; ALMEIDA, L. M. |
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Afiliação: |
FELIPE MENDES ZORZI, USP; LUIZ FRANCISCO ZAFALON, CPPSE; FRANKLIN BISPO SANTOS, Universidade Federal de Alagoas; TICIANO GOMES NASCIMENTO, Universidade Federal de Alagoas; IRINALDO DINIZ BASÍLIO-JÚNIOR, Universidade Federal de Alagoas; ELSA MASAE MAMIZUKA, USP; LARA MENDES ALMEIDA, Universidade Federal de Alagoas; Universidade de São Paulo. |
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Título: |
Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Brazilian Journal of Veterinary Research and Animal Science, v.58, e186701, 2021. |
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ISSN: |
1678-4456 |
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DOI: |
https://doi.org/10.11606/issn.1678-4456.bjvras.2021.186701 |
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Idioma: |
Inglês |
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Conteúdo: |
Fifty-two Staphylococcus aureus recovered from papillary ostium and milk samples collected from cows with subclinical mastitis and milking environments in three small dairy herds located in southeastern Brazil were subjected to PCR identification based on the thermonuclease (nuc) gene. All the strains were submitted to in vitro antimicrobial susceptibility testing, and we investigated the sequence types (STs), agr groups (I-IV), virulence genes encoding for Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs), biofilm-associated proteins, bi-component toxins, pyrogenic toxin superantigens, and enterotoxins. Screening for oxacillin resistance (2-6 μg/ml oxacillin), beta-lactamase activity assays, and PCR for the mecA/mecC genes detected 26 methicillin-susceptible S. aureus (MSSA) and 26 mec-independent oxacillin-nonsusceptible S. aureus (MIONSA). While MSSA isolates were found to be susceptible to all antimicrobial agents tested, or only resistant to penicillin and ampicillin, MIONSA isolates were multidrug-resistant. ST126-agr group II MSSA isolates were prevalent in milk (n=14) and carried a broad set of virulence genes (clfA, clfB, eno, fnbA, fiB, icaA, icaD, lukED, hla, and hlb), as well as the ST126-agrgroup II MIONSA isolated from milking liners (n=1), which also carried the etagene. ST1-agr group III MIONSA isolates (n=4) were found in papillary ostium and milk, but most MIONSA isolates (n=21), which were identified in both papillary ostium and milking liners, were agr-negative and assigned to ST126. The agr -negative and agr group III lineages showed a low potential for virulence. Studies on the characterization of bovine-associated MSSA/MIONSA are essential to reduce S. aureus mastitis to prevent economic losses in dairy production and also to monitor the zoonotic potential of these pathogens associated with invasive infections and treatment failures in healthcare. MenosFifty-two Staphylococcus aureus recovered from papillary ostium and milk samples collected from cows with subclinical mastitis and milking environments in three small dairy herds located in southeastern Brazil were subjected to PCR identification based on the thermonuclease (nuc) gene. All the strains were submitted to in vitro antimicrobial susceptibility testing, and we investigated the sequence types (STs), agr groups (I-IV), virulence genes encoding for Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs), biofilm-associated proteins, bi-component toxins, pyrogenic toxin superantigens, and enterotoxins. Screening for oxacillin resistance (2-6 μg/ml oxacillin), beta-lactamase activity assays, and PCR for the mecA/mecC genes detected 26 methicillin-susceptible S. aureus (MSSA) and 26 mec-independent oxacillin-nonsusceptible S. aureus (MIONSA). While MSSA isolates were found to be susceptible to all antimicrobial agents tested, or only resistant to penicillin and ampicillin, MIONSA isolates were multidrug-resistant. ST126-agr group II MSSA isolates were prevalent in milk (n=14) and carried a broad set of virulence genes (clfA, clfB, eno, fnbA, fiB, icaA, icaD, lukED, hla, and hlb), as well as the ST126-agrgroup II MIONSA isolated from milking liners (n=1), which also carried the etagene. ST1-agr group III MIONSA isolates (n=4) were found in papillary ostium and milk, but most MIONSA isolates (n=21), which were identified in both papillary ostiu... Mostrar Tudo |
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Palavras-Chave: |
Accessory gene regulator (agr); MIONSA; MSSA. |
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Thesaurus Nal: |
Bovine mastitis. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228813/1/VirulenceAgrGroups.pdf
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Marc: |
LEADER 02820naa a2200265 a 4500
001 2137361
005 2021-12-09
008 2021 bl uuuu u00u1 u #d
022 $a1678-4456
024 7 $ahttps://doi.org/10.11606/issn.1678-4456.bjvras.2021.186701$2DOI
100 1 $aZORZI, F. M.
245 $aVirulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis.$h[electronic resource]
260 $c2021
520 $aFifty-two Staphylococcus aureus recovered from papillary ostium and milk samples collected from cows with subclinical mastitis and milking environments in three small dairy herds located in southeastern Brazil were subjected to PCR identification based on the thermonuclease (nuc) gene. All the strains were submitted to in vitro antimicrobial susceptibility testing, and we investigated the sequence types (STs), agr groups (I-IV), virulence genes encoding for Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs), biofilm-associated proteins, bi-component toxins, pyrogenic toxin superantigens, and enterotoxins. Screening for oxacillin resistance (2-6 μg/ml oxacillin), beta-lactamase activity assays, and PCR for the mecA/mecC genes detected 26 methicillin-susceptible S. aureus (MSSA) and 26 mec-independent oxacillin-nonsusceptible S. aureus (MIONSA). While MSSA isolates were found to be susceptible to all antimicrobial agents tested, or only resistant to penicillin and ampicillin, MIONSA isolates were multidrug-resistant. ST126-agr group II MSSA isolates were prevalent in milk (n=14) and carried a broad set of virulence genes (clfA, clfB, eno, fnbA, fiB, icaA, icaD, lukED, hla, and hlb), as well as the ST126-agrgroup II MIONSA isolated from milking liners (n=1), which also carried the etagene. ST1-agr group III MIONSA isolates (n=4) were found in papillary ostium and milk, but most MIONSA isolates (n=21), which were identified in both papillary ostium and milking liners, were agr-negative and assigned to ST126. The agr -negative and agr group III lineages showed a low potential for virulence. Studies on the characterization of bovine-associated MSSA/MIONSA are essential to reduce S. aureus mastitis to prevent economic losses in dairy production and also to monitor the zoonotic potential of these pathogens associated with invasive infections and treatment failures in healthcare.
650 $aBovine mastitis
653 $aAccessory gene regulator (agr)
653 $aMIONSA
653 $aMSSA
700 1 $aZAFALON, L. F.
700 1 $aSANTOS, F. B.
700 1 $aNASCIMENTO, T. G.
700 1 $aBASÍLIO JUNIOR, I. D.
700 1 $aMAMIZUKA, E. M.
700 1 $aALMEIDA, L. M.
773 $tBrazilian Journal of Veterinary Research and Animal Science$gv.58, e186701, 2021.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 2 | |
| 1. |  | ZORZI, F. M.; ZAFALON, L. F.; SANTOS, F. B.; NASCIMENTO, T. G.; BASÍLIO JUNIOR, I. D.; MAMIZUKA, E. M.; ALMEIDA, L. M. Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis. Brazilian Journal of Veterinary Research and Animal Science, v.58, e186701, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|    |
| 2. | | SABINO, A. R.; TAVARES, S. S.; RIFFEL, A.; LI, J. V.; OLIVEIRA, D. J. A.; FERES, C. I. M. A.; HENRIQUE, L.; OLIVEIRA, J. S.; CORREIA, G. D. S.; SABINO, A. R.; NASCIMENTO, T. G.; HAWKES, G.; SANTANA, A. E. G.; HOLMES, E.; BENDO, E. S. 1H NMR metabolomic approach reveals chlorogenic acid as a response ofsugarcane induced by exposure toDiatraea saccharalis. Industrial Crops & Products, V. 140, 111651, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 2 | |
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